>>Can someone please tell me if there is any benefit to using density gradient
>>separation on cells from a lymph node (be it a biopsy or FNA) if you are
>>already using viability gating?
>>What I saw with viability gating looked pretty straight forward, and I
>>couldn't see why any separation media would be needed.
>>I would be interested in any and all experience people have had with the two
>>techniques.
>>Thanks in advance.
>>
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In my experience, it is poor medical practice, unnecessary, time consuming,
and overly expen$ive to pass this type of specimen over a gradient.
>
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Dr. Frederic I. Preffer
preffer@helix.mgh.harvard.edu
Department of Pathology- Cox5
100 Blossom St
Massachusetts General Hospital
Boston MA 02114
(617) 726-7481 {fax x2365}