nuclei preparation

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I have been asked to participate in a study investigating the toxic effects of a
variety of compunds in rats. We want to correlate certain RT-PCR gene patterns
with possible changes in cell cycle patterns.

Basically, I will have access to quick-frozen rat spleen, liver, and kidney from
differnent conditions at various timepoints. Does anyone have their favorite
nuclei preparation method they would like to share? I am interested in those
protocols that have consistant yields of nuclei per mg tissue. I am also
interested in methods that could differentate cells and/or nuclei from different
cell types within the liver or kidney.

I have a Coulter XL-MCL for the acquisition/analysis.

Thanks for all your help!

Marty Giedlin
Chiron Corp.
510-420-6386
510-420-1298 (FAX)

marty_giedlin@cc.chiron.com

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• Previous message: Helene Paxton: "REGISTRATION-THIRD ANNUAL CLINICAL CYTOMETRY SYMPOSIUM"

CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu