Live cell quantification.
James Weaver 301-594-5879 FAX 301-594-3037 (WEAVER@cder.fda.gov)
Fri, 15 Mar 1996 15:25:42 -0400 (EDT)
I would second the idea of using an esterase substrate to measure live cells. I
suggest that calcein-AM is a better choice. It is very bright and is retained
in live cells for many hours. Other labels such as CFDA can be lost in much less
time. Since the emmision is also FITC-like, the possibility for 2-color
live/dead labeling is still intact. Good luck.
-Jim Weaver
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James L. Weaver Email: WEAVER@CDER.FDA.GOV
Molecular Pharmacology Phone: 301-594-5879
Division of Research & Testing FAX: 301-594-3037
OTR, CDER, Food & Drug Admin.
Laurel, MD
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