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Fluorescein diacetate is a dye which is only taken up by live cells. It is
then cleaved within the cell to free the fluorescein resulting in the
staining of only live cells.
Daniel
CDC
Atlanta, GA
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Date: 13 Mar 1996 18:19:06 -0800
From: "Osama Nabulsi" <Osama.Nabulsi@amgen.com>
Subject: Quantifying LIVE CELLS
To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu>
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REGARDING Quantifying LIVE CELLS
I am planning to run a survival bioassay were then I can quantitate LIVE =
cells only using the flowcytometer.
I called Molecular Probes and they suggested "SYTOX" which stains dead =
cells only (but will not cross the membrane of live cells). Have anyone =
used this stain before? Advantages or Disadvantages?
or is there a live cell staining? Suggestions......
Osama Nabulsi
AMGEN INC.
(805)447-1594
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