re: Paraffin-emb. tissue for DNA analysis

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Try using the technique described by Leers et al. (Cytometry 21, 101-107,
1995. This technique combines DNA staining with immunolabeling with
cytokeratin. In this way it is possible to select for epithelial (tumor)
cells in your samples.

Bert Schutte

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu