Re: Re : platelet prep
T. Vincent Shankey (tshanke@bsd.meddean.luc.edu)
Thu, 29 Feb 1996 21:15:07 -0600 (CST)
Walter,
Regardiing your comments on analysis of whole blood using antibodies to
GP IIbIIIa (CD41, 42a or 61)- why do you lyse the RBC's? We routinely
perform whole blood flow analysis of platelet activation (in vivo or in
vitro) without any lysis step. I would be concerned about RBC ghosts
which may have similar FALS to platelets, and I don't see any advantage
to lysing.
Vince Shankey
Loyola niv. Med. Cntr.
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