Requestion information on chymopapain - reply
Antony Bakke (bakkea@ohsu.EDU)
Mon, 26 Feb 96 08:59:37 PST
Any protease will remove some of the surface markers on the cell.
This is a significant problem when working with cell lines that
attach to the surface of the culture dish. We have used EDTA and
mechanical disruption to make cell suspensions instead of proteases.
Some surface antigens will be re-expressed on the cell surface
within a few hours, but the cells often clump or attach in this time
also, putting you back where you started.
Hope this helps,
Tony Bakke
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
