5 Color Surface Phenotyping

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Is anybody out there doing five color surface immunophenotyping by flow
cytometry? If so what fluorochromes are you using?
My strategy is FITC, PE, Red613, Cy-X, and a UV excitable dye such as Casade
Blue or AMCA. How good are these dyes (Casade Blue & AMCA) ? Are there
any other dyes available for a fifth color - my cytometer is equipped with
488, 633, and multi-line UV laser. APC is out of the question, since Red613
(PE-Texas Red Tandem) dyes are weakly excited by 633 line . Any suggestions
will be useful.
Thanks in advance.
Regards,
O. Joseph Trask, Jr.
The Ohio State University Comprehensive Cancer Center
223 Wiseman Hall, 400 West 12th Avenue
Columbus, Ohio 43210
Tel: 614/292-FLOW(3569)
Fax: 614/292-7335
E-Mail: cytometry@osu.edu

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu