RNA Extraction from fixed sorted cells ?

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I would like to know the special tricks to use, if one sorts fixed human
cells for RNA / DNA / Protein extraction afterwards. I think because of
fixation (which has to be done for security reasons), there could be a
problem of RNA degradation etc... Do you add RNAse, DNAase or Protein
inhibitors to the samples ?

I know that this subject was probably already on the subject list, but I
don't have good access to the archives (from Europe it's quite slow).. so I
would still be interested in getting some hints.

thank you very much,

Matthias

______________________________________________________________
Matthias Haury _/_/_/ _/_/_/ _/_/_/
Flowcytometry - Immunology _/ _/ _/ _/ _/
Institut Pasteur Paris _/ _/_/_/ _/_/_/
Email: mhaury@pasteur.fr _/ _/ _/
Tel: 33 (1) 40 61 31 29 _/_/_/ _/ _/
Fax: 33 (1) 45 68 86 39 INSTITUT PASTEUR PARIS
______________________________________________________________

• Next message: Ray Hicks: "Mozilla"
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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu