Flow cytometry vs. Immunoblotting and Enhanced Chemiluminesence

Alan Salkind (nepsy@umsmed.edu)
Wed, 14 Feb 1996 10:57:23 -0600

What are the relative attributes of detecting antigens (specifically those
that are intracellular) by flow cytometry vs. ECL? In our system, we've
found immunoblotting and ECL to be much more sensitive. Does anyone know
the limits of antigen detection of flow vs. ECL?

Thanks in advance for your comments and ideas.

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu