enhanced chemiluminescence vs. flow cytometry

Alan Salkind (nepsy@umsmed.edu)
Tue, 13 Feb 1996 14:56:05 -0600

We observe relatively small changes (approximately 10%) in the mean
fluorescence intensity of an intracellular antigen by flow cytometry.
However, when we analyze cell lysates by immunoblotting and ECL the signal
is significantly increased. Is this a problem of comparing apples with
oranges? Or is ECL much more sensitive for detecting small changes in
antigen levels?

Thanks in advance for your comments and ideas.

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