Re: Macrosort, chromosomes
John J. Fawcett (fawcett@lanl.gov)
Tue, 13 Feb 1996 09:49:36 -0700
On 8 Feb96, Roger Schultz writes:
>To chromosome sorters:
>
>We're using the BD Macrosort on FACStar Plus for chromosome sorting.
>Experienced significant FSC background which was corrected with a wider
>obscuration bar. Now with drop drive on experiencing significant SSC
>background. BD does not recommend wider obscuration bar for SSC. Looking for
>opinions on Macrosort option and specific suggestions re: background.
>
>Roger Schultz, schultz@ryburn.swmed.edu
Roger,
We have two Coulter instruments (EPICS V, EPICS 752) devoted to
chromosome analysis and sorting. Each is equipped with "sense in quartz"
fast flow tips.
Chromosome resolution is strongly influenced by the amplitude of
the droplet drive. It has been our experience that it is extremely
important to find tips which make droplets efficiently.
For our needs, only the quantification of fluorescence is required
(no FSC). FSC is not measured and no obscuration bar is used.
Joe Fawcett, fawcett@lanl.gov
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
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If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
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EMAIL robinson@flowcyt.cyto.purdue.edu
