Re: Compensation versus antibody interactions
Howard Shapiro (hms@shapirolab.com)
Mon, 12 Feb 1996 20:52:37 -0500 (EST)
>>What happens to the compensation if you are running two antibodies (one
>FITC, one PE) which are interacting with each other or are recognising the
>same antigen?
Energy transfer from FITC to PE would be expected to increase the
yellow-orange and decrease the green fluorescence signal. The PE signal
would then look undercompensated, while the FITC signal would look
overcompensated. Compensation of the conventional type assumes that there
isn't significant energy transfer; how to compensate when there is is a
philosophical question.
--Howard
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