Compensation versus antibody interactions

Jenny Bryant (jbryant@nsw.redcross.org.au)
Mon, 12 Feb 96 04:58 EST

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>What happens to the compensation if you are running two antibodies (one
FITC, one PE) which are interacting with each other or are recognising the
same antigen? I'm looking at autoantibodies against platelet antigens using
an anti-CD41-PE and an anti-human Ig-FITC. I can set my compensation
correctly on some samples but others (which may have weaker fluorescence)
behave as though no compensation has been performed.
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Jenny Bryant
Flow Cytometry Laboratory
Department of Cell Biology
NSW Red Cross Blood Transfusion Service
Sydney, NSW 2000, AUSTRALIA
Ph: 61 2 229 4341
FAX: 61 2 229 4372
Email: jbryant@nsw.redcross.org.au

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