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Stephen Perfetto
Department of HIV Disease Prevention
Walter Reed Army Institute of Research
1600 East Gide Drive
Rockville, MD. 20850
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Subject: Indo-1 Ratio Mean channel c
Author: Susan Grigsby <susan_grigsby@iucc.iupui.edu>
Date: 5/10/95 10:08 AM
5/10/95 9:40 AM
IUCC Re: Indo-1 Ratio Mean channel calculations
Good Morning Flow Fanciers,
We have a FACStar Plus/ HP Lysis II that we use to acquire ratios of free and
bound calcium vs time for an invesigator. Does anyone know of a program we
can use to calculate mean channel ratio vs time and percent responding cells
from our stored data? It would also be helpful to know how we might save
dotplots instead of listmode files on the HP. This would enable us to use our
Cicero V. 3.10 to make these calculations. We have tried to accumulate the
data directly in Cicero, however; we are simultaneously collecting our data
plus a low channel ratio range over time. This noise range is calculated as
50% of our responding cells in Cicero while the HP graph shows that 100% of
our cells are responding. I hope this makes sense and someone of you have a
solution. Thanks.
Susan Grigsby
Indiana University Cancer Center
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