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We have similarly found a wide "normal" range in platelet Ig measurements by
flow.
It seems that most of the current literature suggests that measurement of
platelet-associated IgG by flow cytometry has about the same utility as
other methods (plate assays etc.) for examining PAIgG, i.e. fair sensitivity
but relatively poor specificity (see reviews by KA Ault). There is some
literature on measurement of platelet RNA (reticulated platelets) by flow
cytometry suggesting that an increased percentage of reticulated platelets
is a specific sign for increased marrow production and indirectly
therefore for increased platelet destruction. Perhaps a combination of
reticulated platelets and PAIgG by flow will be most specific.
From: H. Rinder
Henry.Rinder@Yale.edu
New Haven, CT, USA
Ph. 203-785-4231
Fx. 203-737-4111
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From: owner-cyto-sendout
To: cyto-inbox
Subject: ANTI-THROMBOCYTE ANTIBODIES IN ITP
Date: Monday, May 08, 1995 10:13AM
WE HAVE BEEN LOOKING INTO THE POSSIBILITY OF USING FLOW CYTOMETRY TO DETECT
THROMBOCYTE BOUND IG ON PATIENT THROMBOCYTES AND THE BINDING OF PATIENT
SERUM IG TO CONTROL THROMBOCYTES BY FLOW IN ITP.
WE FOUND, ESPECIALLY IN THE DIRECT STAINING OF THROMBOCYTES, QUITE A GREAT
VARIABILITY ALSO IN "NORMAL CONTROLS".
WE ARE USING FRESH THROMBOCYTES ENRICHED BY CENTRIFUGATION AND ARE STAINING
LOG SCATTER-GATED THROMBOCYTES IN SINGEL COLOR ANALYSIS FOR HUMAN IG-G AND
IG-M.
I FIND OUR RESLUTS "LESS THAN OPTIMAL!!"
IS THERE ANYONE WHO BELIVES THAT FLOW CYTOMETRY IS A CLINICALLY USEFUL
METHOD FOR THIS ANALYSIS IN ITP??
ARE THERE ANY GOOD PROTOCOLS AVAILABLE AND IS THERE ANY FOLLOW UP AS TO THE
SENSITIVITY AND SPECIFICITY OF THIS OR OTHER THROMBOCYTE TESTS IN ITP??
-------------------------------------------------------------------------
Birger Christensson, MD, PhD
Dept. of Pathology, F49
Huddinge University Hospital,
S-14186 Huddinge,
SWEDEN,
Tel +46-8-7461000
Fax +46-8-7795520
bich@PATD01.HS.SLL.SE
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