Re: simultaneous surface/DNA analysis
FACS_COPY@wehi.edu.au
Mon, 01 May 1995 13:41:56 +1000
On the subject of surface vs DNA analysis: unfortunately I have no
good suggestions to add but would like to comment on a couple of
suggestions made so far. The problem of PI (DNA) vs PE (surface) is
that the intense PI fluorescence leaks into the PE detector. Nothing
you do to the PI detector optical filters will change that; going to
a longer wavelength PI filter doesn't change the PI emission spectrum.
The PE detector will be subjected to just the same amount of
leakage. On the other hand, going to a *shorter* wavelength *PE
detector* optical filter may indeed take you into an area of better
PE-to-PI detection ratio.
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