U937 & CD20

Thomas Delohery (t-delohery@ski.mskcc.org)
Thu, 27 Apr 1995 12:28:31 +0530

Hi, we're having problems with non-specific binding of anti-CD20 to
U937 cells. BD's @CD20-FITC works fine but @CD20-PE has high
background (supposedly the same clone). MAb from different
clones obtained from Caltag and Coulter also have high background
relative to isotype controls.
Can anyone make a recommendation on a MAb source?
Any help would be greatly appreciated.

tom d.

--
==============================================================================
 Thomas Delohery                        | Internet: t-delohery@ski.mskcc.org
 Manager, Flow Cytometry Core Facility  |    Phone: (212) 639-8729
 Memorial Sloan-Kettering Cancer Center |      Fax: (212) 794-4019
==============================================================================

Home Page Table of Contents Sponsors Web Sites
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu