DNA content of yeast cells

DAVIN B. JUTILA (SM0973%SPRINGB.SIUCVMB@SIUCVMB.SIU.EDU)
Tue, 25 Apr 95 15:32:00 CST

*-------------------- FLOW CYTOMETRY FACILITY

Hello from Lincoln Land.

I was wondering if anybody has, or knowledge of, a procedure to analyze
DNA content of yeast cells of Histoplasma Capsulatum, Candida and
Saccharomyces. So far all the article I have seen have iffy histograms.
The investigator also would like to see if a view of apoptosis is possible?
Are the procedures in Methods of Cell Biology for apoptosis applicable?
Also any suggestions on, if these cells should be ran on a cytometer.
And if so, what kind of decontamination procedure should be used.
Any help will be greatly appreciated. Thank you in advance.

DAVIN JUTILA, DIRECTOR SIU SCHOOL OF MEDICINE
FLOW CYTOMETRY FACILITY-1220 SPRINGFIELD, IL 62702
PHONE: (217)-524-5812 FAX: (217)-524-3227
EMAIL: SM0973%SPRINGB@SIUCVMB.SIU.EDU

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