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A problem has come up with one of our grad students using the ACAS 570
confocal laser scanning microscope. He is looking for the oxidative burst
in live cultured macrophages using:
dihydrorhodamine --H2O2, enzymes-->rhodamine 123
He used to get good rhodamine 123 fluorescence. Then he changed the cell
culture conditions - mainly added Pen/Strep and Nystatin to hold down
contamination. Now he gets little or no rhodamine 123 flourescence. We're
wondering if the addition of antibiotics to the culture medium could be the
cause, or if there are other pitfalls in this method. If any of you have
any experience with this technique, we would appreciate your input.
TIA
* * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * *
* Richard K. Meister Email: Meister.1@osu.edu *
* The Ohio State University Voice: (614) 292-9716 *
* Dept. of Veterinary Biosciences FAX: (614) 292-6473 *
* Cytometry Instrumentation Lab *
* 1925 Coffey Road *
* Columbus, OH 43210 U.S.A. *
* * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * *
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