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> We are trying a saponin permeabilization technique - to measure
> intracytoplasmic cytokines and other proteins (p24 etc). We are
> initially fixing with 4% formaldehyde and then coming in with saponin
> and then labelling with our Ab.
Final conc 4% or 2%? Our protocol uses equal volumes of cells and 4%, i.e.
2% final, slightly hypertonic. Duration of fix and temperature can also be
critical.
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*Peter Openshaw p.openshaw@ic.ac.uk *
*Respiratory Medicine Tel:+44 171 723 1252 x 5781/6 *
*St. Mary's Hosp. Med. School Fax:+44 171 724 7349 *
*Imperial College, Norfolk Place, London W2 1PG, UK *
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