Beta-amyloid precursor protein antibodies

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A few years ago we did a lot of work labelling a ras oncogene product p21 with afluorescein conjugated antibody. We found that fixation produced quite a bit ofbackground. We ultimately used lysolecithin to permeabilize the cells, which did not result in
much background, and got nice histograms with many intact cells. We also have used
electroporation to open pores in the plasma membrane, allowing antibody to pass
with cells still viable. Worked great! I would suggest conjugating your antibody

Debbie Berglund
Microbiology
Montana State University

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu