Re: Setting up indo-1

Marty Bigos (BIGOS@Darwin.Stanford.EDU)
Tue, 11 Apr 1995 07:53:55 -0700 (PDT)

Regarding filter setup for Indo-1, the following filters have been used
successfully here for quite a while:

beam splitter: 460 shortpass dichroic
UV violet : 405/20 bandpass
UV green: 537/45 bandpass
Filters were purchased from Omega Optical.

Smith could verify that the filters used are adaquate for detection by removing
the beam splitter and manually switching each filter into "FL4", which I am
assuming is the "straight-through" channel. If signal is measurable, then one
could suspect alignment of the dichroic or FL5 PMT.

-Marty Bigos
Stanford Shared FACS Facility


Home Page Table of Contents Sponsors Web Sites
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu