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> PROBLEM: Profile data does not appear to contain any equivalent to the FCS
> "$TOT" keyword; That means that all the analysis software at my disposal
> cannot analyse the files, because they appear to contain NO events!
You're right, Pro2FCS files do not provide $TOT (in any FCS form).
I've modified MFI to work fine with them, though. Here's an excerpt
from a beta-test announcement for MFI I made on 1/13/95. (Subsequently,
MFI 3.4I has been released including these features.)
[BEGIN QUOTE]
A peculiarity of this [Pro2FCS] file structure is the absence of $TOT. I
calculate the total number of events from the parameter data sizes and the
offsets. If anyone is interested, ask and I can make available the C code
I use. I do this for all files anyway since in some cases the number of
events actually recorded in the data block is less than that given in
$TOT. [A warning message is emitted when there's a discrepancy.] I have
not been able to obtain a Pro2FCS file containing TIME as a parameter so
MFI may not interpret the TIME range properly when it first encounters such
a file.
[END QUOTE]
I still have never obtained a Pro2FCS file with TIME. If someone has
such, please put a sample in /incoming at flowcyt.bio.umass.edu for
my and MFI's edification.
/*- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
Eric Martz, Professor of Immunology emartz@microbio.umass.edu
Dept Microbiology Voice: 413-545-2325 FAX: 413-545-1578
Morrill IVN 203, Box 35720, Univ Massachusetts, Amherst MA 01003-5720
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -*/
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