Re: Fluorescence intensity from large cells
Larry Seamer (larry@athena.unm.edu.unm.edu)
Thu, 6 Apr 95 09:30:30 GMT-0800
Dave Coder's response neglected one key aspect of Bjarne's original
question. It appears from the question that Bjarne is acquiring
*peak* fluorescence signals. However, Absolute fluorescence (e.g.
receptor number) is best measured on *integrated* fluorescence
signals, where the size of the cell (compared to the laser beam) is
not a factor. Newer FACScans and, I suspect, the FACSort have the
capacity to measure integrated fluorescence. With that caveat, I
agree with Dave's suggestions.
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
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If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
