sorting cells for RNA

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I will soon be sorting cells to obtain RNA from sorted cell populations. I
was wondering if anyone had dealt with such issues as rinsing the lines with
RNAse inhibitors, sorting directly into solutions containing RNAse
inhibitors, keeping the instrument cold, etc. We use a BD FACStar Plus. I
believe Peter Openshaw from St. Mary's Medical School asked essentially the
same question 4 months ago, with more emphasis on somehow preserving the
cells for post-sort analysis. I do not yet know how imperative that issue
will be for this project but any suggestions or insights would be gratefully
appreciated. Please send replies to tstaley@amgen.com.

Tabitha Staley
Amgen, Inc.
Thousand Oaks, CA
FAX (805) 447-1982
TEL (805) 447-1196

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu