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In reply to your question about plasma cells in marrow, and the use
of a method for light chain measurement.
We have tried an antibody advertised for plasma cells (B-B4). To
determine specificity for B cells, different B cell malignancies
(B-CLL, non-Hodgkin's lymphomas) have been tried. They have been
consistently negative.
Recently, we had a case involving blood, marrow, and scalp lesion. We
were able to show that this marker was positive on surface labelling,
but could not demonstrate monoclonal kappa or lambda. We do not
ficoll for our surface immunoglobulins, but rather use a commercial
lysing system after a PBS wash, then label 3 colour with no problems.
Using a commercial permeablizing agent, cytoplasmic heavy chains and
light chains were able to be demonstrated,ie IgG+(kappa+), IgA+, and
show B-B4+, & CD19-.
The heavy and light chains were confirmed by immunohistochemistry.
The cells were morphologically like plasmacytoid lymphocytes as
opposed to mature plasma cells.
We have had no further myeloma cases to date. Sorry I didn't run a CD
38 as suggested by Kenneth Ault.
Paul Harris
St Joseph's Health Centre
268 Grosvenor St.
London, Ont. Canada
ph (519) 646 6100 5918
fax (519) 646 6088
paulh@stj.stjosephs.london.on.ca
>>>>>>>>>>>>>>>
I have a question about a the possibility to quantitate myeloma cells
in bone marrow samples, preferably without initial gradient
separation.
I would like to be able to stain for light chains in the cytoplasm
and at the same time use another plasma/myeloma cell marker to
distinguish specific from non-specicfic light chain staining. The
problem is compounded by the difficulty in using scattergating for
the identification och myeloma cells.
Has anyone solved this problem or can recommend any other method for
enumerating myeloma cells in bone marrow samples?
Birger Christensson
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Birger Christensson, MD, PhD
Dept. of Pathology, F49
Huddinge University Hospital,
S-14186 Huddinge,
SWEDEN,
Tel +46-8-7461000
Fax +46-8-7795520
bich@PATD01.HS.SLL.SE
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