DNA analysis of insects

cheryl@immune.med.utoronto.ca
Mon, 27 Mar 1995 12:02:06 EST

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Hello out there!
I have a user who wishes to do ploidy analysis on various species of carion
consuming flies. The hope is to compare DNA content and possibly identify
cryptic species using PI staining. Has anyone had experience in this area?
What would be the best method of accessing the DNA in the hemolymph and are
there any tricks to the staining (tonicity of solutions, etc.)?

Hope Someone can help....Thanks
Cheryl Smith
Dept. of Immunology
University of Toronto

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu