Re: DAPI/BrdU
David Hedley (70740.1375@compuserve.com)
23 Mar 95 14:41:39 EST
Yes, the answer is no. First, DAPI binds to DNA via two modes; the first
being intercalation between base pairs and the second an outside binding
to the minor groove at AT rich sequences. Second, DAPI also binds RNA.
The fluorescence seen is the sum of this three modes of binding and
consequent fluorescence enhancements. The quenching of fluorescence by
BrdU incorporation in one mode is canceled out by the fluorescence
yielded by the two other modes. There has been a number of papers on
these DNA / RNA binding modes of DAPI in Biochemistry in the last few
years (which I now do not recall in detail). If you would like to have
more information on DNA staining please direct your questions to :
martin@probes.mhs.compuserve.com (Martin Poot at Molecular Probes, Inc.,
Eugene, OR 97402).
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
