Fluo-3 on fibroblasts

Hank Pletcher (pletcher@MSCF.MED.UPENN.EDU)
Tue, 21 Mar 1995 16:24:12 -0500

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Does anyone have any experience loading non-hematopoetic cells,
particularly fibroblast cell lines, with Fluo-3? Most of the literature
seems to be on lymphocytes or neutrophils. We would appreciate any
references; or suggestions on loading, controls (+/-), ionophores, and
pitfalls (temperature, etc.)
Thanks in advance.

Hank Pletcher (pletcher@mscf.med.upenn.edu)
University of Pennsylvania Cancer Center

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu