membrane and nuclear protein
Antony Bakke (bakkea@ccmail.ohsu.edu)
Tue, 21 Mar 95 09:49:38 PST
Becton-Dickinson has a method for three color labeling for cell
surface antigens, cytoplasmic antigens and DNA content. This
could be modified to allow staining of cell surface and nuclear
antigens. I have not tried this particular method, but I have
labeled two surface antigens and DNA. You must do the surface
labeling first before fixing. The reference B-D gives is
Schmid, Uittenbogaart and Giorgi. A gentle fixation and
permeabilization method for combined cell surface and
intracellular staining with improved precision in DNA
quantification. Cytometry 1991; 12: 279-285. You could ask
your local B-D rep for a copy. Also Caltag makes a simple Fix
and Perm kit that should work for this purpose. Other companies
have similar products, or your can make your own reagents.
Good luck. Tony Bakke (bakkea@ohsu.edu)
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
