BrdUrd alternative method
darzynk@nymc.edu
Thu, 16 Mar 1995 10:30:43 -0500
Dave Coder have inquired about the method that can be used to detectd
BrdUrd incorporation and be compbined with immunophenotyping. We have
developed the method ofthe detection of BrdUrd incorporation which
does not require DNA denaturation. The method is based on selective
DNA strand break induction by photolysis (SBIP) followed by labeling
the resulting strand breaks with fluorochrome conjugated
deoxynucleotides by terminal transferase. The method appears to be of
comparable sensitivity with the immunocytochemical detection of
BrdUrd. It was described in: Li et al., Int. J. Oncol. 4:1157, 1994
and one of its applications in: Li et al., Cancer Res. 54:4289,
1994.
The modification and further improvement of this technique will be
presented in one of the forthcoming issues of Cytometry.
Zbigniew Darzynkiewicz
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
