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Howdy Flow Folks,
We have just started to use the PerCp fluorochrome in a 2 color immunophenotype
panel. The combination is Alpha Beta FITC/ CD4 PerCp and Gamma Delta FITC/
CD4 PerCp. My knowledge of PerCp is limited and only remembered alittle
of what was said about it on the network. I remembered that PerCp photobleached
in jet-to-air sorters that had a high laser power. I have ran Human lymphocyte
with the fluorochrome at a detuned laser power of 20-30mW (488nm line) which
was recommended by the manufacturer. The plots and percentages looked okay, so
I took a look at the signal at a higher laser power. I retuned the laser so it
was operating optimally and lowered the power to it's limit which was 130mW.
The corresponding PerCp signal actually looked better and with better
separation. The percentages look the same as they did with the lower power.
I did see an increase in the noise level in the FL3 detector when PE was ran.
So I guess my question is: Can PerCp be routinely detected at higher laser
powers? or is there something I'm missing. Any responds from individual with
experience with PerCp (laser power, instrumentation, etc) would be greatly
appreciated.
Take care...
DAVIN B. JUTILA *--------------* SIU SCHOOL OF MEDICINE
FLOW CYTOMETRY FACILITY - 1220 SPRINGFIELD, IL 62702
PHONE: (217)-524-5812 FAX: (217)-524-3227
EMAIL: SM0973%SPRINGB@SIUCVMB.SIU.EDU
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