live human cell sorting
system@flocyt.int-med.uiowa.edu
Mon, 13 Mar 1995 16:13:31 EST
We are attempting to modify our policy dealing with sorting
live human samples. Our current policy allows sorting live
human samples (PBL's) that are tested for HIV, but the
testing is done after the sort. All of the usual precautions are
taken during the sort as outlined in the (scant) literature
including gowning, face shield, gloves, biohazard
containment sort chamber, religious sorter cleaning with
10% bleach, etc. There are still a few questions that we have
not resolved-
1) Even though all samples are handled as if they are
infected, should samples known to be positive for either HIV
or hepatitis be sorted?
2) Should human derived cell lines be treated the same as
those coming from patients?
This cell line question seems to me to be particularly messy
because of the time and cost involved in testing and cleanup
if every LIVE human derived cell line is treated as if infected
when run on the cytometer.
3) Is there a governing body (ISAC?, etc.) that has written
guidelines specifically targeted at flow cytometry?
I realize some of this has been discussed before on the net
and that basically there was no consensus. As usual, these
questions are driven by investigators wanting to run
experiments for which there is no policy.
Justin Fishbaugh
University of Iowa
Flow Cytometry Facility
internet: justin-fishbaugh@uiowa.edu
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If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
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