Acridine Orange

Gurnsey, Michael (GURNSEYM@agresearch.cri.nz)
Fri, 10 Mar 1995 15:49 +1200 (NZST)

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We are interested in using AO to look at DNA damage resulting from sperm
sorting. I am aware from this group that AO binds well to some tubing and
have seen various methods of cleaning this up.

Specifically, I am a guest user on a Profile II in a busy Haematology Dept.
I don't want to make myself unpopular and unwelcome. I am told that use of
alcohol would not be good for polycarbonate parts in the Profile. Will 50%
bleach for 10 min followed by water do the trick? Any one had any
experience with this problem? If the worst comes to the worst I guess I
could retube. Is this a difficult operation in the Profile?

Thanks in advance for your help.

Regards, Michael Gurnsey

Dr Michael Gurnsey
AgResearch Ruakura
Hamilton
New Zealand

Email gurnseym@agresearch.cri.nz

Next message: Geoffrey Osborne: "Mac Tutorials Available"
Previous message: Matthew J Shaw: "Alternative cytometry hardware and methods"

CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu