Re: Apoptosis model systems
Mike Salmon (salmonm@rheuma.bham.ac.uk)
Wed, 8 Mar 1995 12:13:12 +0000
Dexamethasone is not a good way to induce apoptosis in peripheral
human T cells. Our standard control for many assays is very simple:
Mouse CTLL2 cells are readily available and are very highly dependant
on IL-2. 24 hours before you wish to do the experiment wash some of
these cells and put them back into culture with IL-2 (negative
control) or without IL-2 (positive control). This is extremely
reliable, but you may need to ficoll the cells first if you don't
split them often enough, or your cultures will all contain apoptotic
cells.
Have fun
...........................................................
Mike Salmon
Department of Rheumatology
The Medical School
University of Birmingham
Birmingham B15 2TT
United Kingdom
Tel: 44 (0)121 414 6780
Fax: 44 (0)121 414 6794
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