Fish Lymphocytes:DNA Histograms

Ross Longley (Longley@HBOI.EDU)
Mon, 06 Mar 95 15:09:00 PST

Thanks to all regarding how to isolate fish lymphocytes. The old tried and
true "experiment with diluting ficoll hypaque" worked out after all! Now
here is the other most interesting problem. Before I was able to isolate
the lymphocytes, I took a look at the "buffy coat" by staining with P.I. and
looking at the resulting DNA histograms. I got something quite interesting.
The RBC's which are nucleated in the fish, gave what I considered to be a
"normal" G1-S-G2/M histogram pattern--but further on down the fluorescence
scale--I saw another G1-S-G2/M pattern. Is this indicative of WBC's in the
fish i.e. does the DNA of WBC's stain more intense than the DNA of the
RBC's?

Help!!

--Ross--

Home Page Table of Contents Sponsors Web Sites
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu