Re: Dim Fluoresscence
Dennis_Young@CIS.ucsd.edu
Wed, 1 Mar 1995 15:34:00 -0800
1) The subtraction option in Lysys II can show the channel per channel
differences in your sample.
2) Kolmogorov-Smirnov is available (It's even Russan :) (Young, IT, Journal of
Histochemistry and Cytochemistry, Vol. 25, No. 7, pp. 935-941, 1977) (NO
relation).
3) Another "quick and dirty" way is to overlay the control and set the marker at
the intersection of the curves. With dim samples, this can be as much as 20% of
the control! (Chapter 5.2.2 , Supplement 4 Current Protocols in Immunology,
1992)
The *MOST* important problem with dim fluorescence is that you *WILL* see
differences even when they are purely due to natural variability. The binding
should confirmed by other methods.
There are also numerous ways to amplify your signal.
*****************************************************************************
* Dennis J. Young Voice : (619) 543-3928 *
* Flow Cytometry Core Facility FAX : (619) 543-7487 *
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