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I would like to thank all the people who responded to my question about
Hoechst 33342 and FITC staining, it helped greatly.
At the end of this month I will be giving a seminar on some of the applications
of our FACS Vantage to the facility here. One of the methods I wanted to
discuss was the chromosome karotyping with Hoechst 33258 and Chromomycin A3.
So far the papers read have said that chromomycin A3 is excitable with a
argon ion laser tuned to a 457.9nm line. My question, is our enterprise
argon ion laser from Coherent able to be tuned to a 457 line? We have model
621. Or are we in need of another laser if we ever start to sort chromosomes.
If my laser is unable to excite the DNA dyes that are excitable at a 457 line,
are there alternatives? If the laser can be tuned, how?
Thank you in advance.
DAVIN B. JUTILA *--------------* SIU SCHOOL OF MEDICINE
FLOW CYTOMETRY FACILITY - 1220 SPRINGFIELD, IL 62702
PHONE: (217)-524-5812 FAX: (217)-524-3227
EMAIL: SM0973%SPRINGB@SIUCVMB.SIU.EDU
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