Re: degranulated polys/light scatter

Dave Coder (dave@nucleus.immunol.washington.edu)
Wed, 11 Jan 95 09:01:29 -0800

The forward light scatter of PMNs increases substantially (both in magnitude
and range) following exposure to fMLP. We noticed this quite some time back
in measuring calcium flux of PMNs. We didm't look at myeloperoxidase levels,
however.

Dave Coder
dcoder@u.washington.edu

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Date: 10 Jan 1995 17:47:15 -0500 (EST)
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From: helix.mgh.harvard.edu@HELIX.MGH.HARVARD.EDU (Frederic Preffer)
Subject: degranulated polys/light scatter
To: cytometry@flowcyt.cyto.purdue.edu
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has anyone experience with the light scatter characteristics of degranulated
neutrophiles? are these cells still myeloperoxidase positive?
`````````````````````````````````````````````````
Frederic I. Preffer
preffer@helix.mgh.harvard.edu
Department of Pathology- Cox5
100 Blossom St
Massachusetts General Hospital
Boston MA 02114
(617) 726-7481 {fax x2365}
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