Re: Staining with H33342 and FITC
Hector Nolla (hector@soest.hawaii.edu)
Thu, 5 Jan 1995 08:45:08 -1000 (HST)
We routinely use hoechst(?) for staining bacterial DNA in enviromental
marine samples. Our experience has been that it is the hoechst
fluorescence which overlaps with FITC fluorescence. I think I remember
comparing the emission spectra of both and it seemed that hoechst dyes
can have a wide but dim tail of emission that overlaps with the much
brighter FITC emission. You may be able to resolve both fluorescence
better using a 2 parameter histogram that displays Log green and Log blue
fl(maybe). You could also test if FITC is excited by the UV laser by
blocking the 488nm while running your sample. I hope this helps.
Hector Nolla
Department of Oceanography
University of Hawaii
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