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John W. Brandes
stickboy@ix.netcom.com
(617) 639-8760
Marblehead, MA
USA
Resume Follows:
John W. Brandes
4 Hines Way
Marblehead, MA 01945
(617) 639-8760
Objective: Secure a position in a research, clinical, or diagnostic environment
that would allow me to take advantage of my experience in immunology
and flow cytometry.
Qualifications: Proficient in a research lab situation using flow cytometry.
Extensive practical experience in immunology using monoclonal
antibodies, microbiology techniques, and molecular biology. Trained
in the construction and repair of flow cytometers; both commercial
and custom built. Trained in hardware and software repair of personal
computers. Experience in the Windows, DOS and Macintosh platforms,
working knowledge of C, FORTH, and BASIC programming languages.
Member in good standing of the International Society for Analytical
Cytology (ISAC).
Technical Positions Held:
Jan. 1993-June 1994 Howard Shapiro, M.D., P.C., 238 Broadway, Cambridge, MA
Job Title: Research Technologist
Constructed prototype flow cytometer
Performed undergraduate research project
Assisted in development/update of 4Cyte flow cytometry
software in FORTH
Collaborated on the third edition of Practical Flow Cytometry
with Dr. Shapiro
May 1992 - Aug. 1992 Coulter Corporation, 600 W 20th Street, Hialeah, FL
Job Title: Research Technologist
Assisted in the development of product improvements:
*Selection and optimization of optical filters
*Evaluation of Coulter Q-PrepTM fluidics
*Modifications to flow cell positioning and orientation
Participated in new product development:
*SpheriCalTM for absolute counts on ProfileTM flow cytometer
*Three color CD3/CD4/CD14 assay
Jan. 1992 - May 1992 Howard Shapiro, M.D., P.C., 238 Broadway, Cambridge, MA
Job Title: Research Technologist
Learned flow cytometer design theory and techniques:
*Flow cell design
*Configuration of optics
*Laser selection and focusing
*System optimization
May 1991 - Aug. 1991 Coulter Corporation, 600 W 20th Street, Hialeah, FL
Job Title: Reagent Technical Support Specialist
Developed operational proficiency:
*Coulter ProfileTM II flow cytometer
*Becton Dickinson FACScanTM flow cytometer
Performed comparative evaluation of monoclonal antibodies:
*Four different manufacturers evaluated
*Report used to develop new marketing and sales strategies
Foreign Language: Working knowledge of Spanish
Education: Boston College, Chestnut Hill, MA
B.S., Biology, 1994
References: Available upon request.
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