FACScan vs EPICS

Susan D. DEMAGGIO (SDDEMAGG@uci.edu)
Thu, 22 Dec 94 08:34:54 PST

Fellow Cell-Mates

I wondered if this is a common problem and what might be the solution. I just
attempted a sort for someone who found a population of expressing T cells (Don't
know what protein) of about 8% positive green fluorescence by FACScan analysis
(he did it at his facility so I don't know anything about how bright they were.)
I couldn't identify them anywhere on the EPICS (I admit it is old and the laser
is LAZY - but they should have been there - shouldn't they have??) I'd like to
help him (and my facility needs the money!!). Any tips on how to identify that
population? Or is my sorter just not as sensitive as the SCAN??

SUE DeMaggio
UC Irvine

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