Re: Multi Well Fluorescence Plate Readers

(no name) ((no email))
Fri, 16 Dec 1994 15:31:13

Subject: Multi Well Fluorescence Plate Readers

I have a Perkin Elmer LS50 B with a microplate reader attached by
fiber optic.
It is in the core facility and anyone can use it if they want to. We
compared it to several dedicated fluorescence plate readers and it
was OK - but not quite as good as most. This may be because it reads
from the top of the plate not the bottom. We do use it, and it does a
reasonable job. Since it only cost a few extra $K it was considerably
cheaper than a stand alone reader and we were buying the LS50B
anyway. It is easy to use, but the data collection and analysis is a
bit cryptic.....

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Author: <gebhard@aecom.yu.edu>
Date: 12/13/94 18:46

I have recently been asked to "recreate" by flow some experiments
originally performed on multiwell fluorescence plate readers(MWFPR), (with
mixed success).

The thought occured to me that as a Core FACS Facility, specializing
in fluoresence analysis that a useful service/technology to offer and
support would be a MWFPR. It is a first cousin to FACS, and I think would
be a popular and useful adjunct to the FACS Core.

Question, does anyone have any practical experience with this kind of
device? Any caveats? Are there any FACS Cores now offering this
technology? Is there a model/system that is particularly well thought of?
Dave Gebhard
Albert Einstein College of Medicine PH: 718-430-3573
FACS Facility,Oligo Facility, FAX: 718-822-6538
DNA Sequencing Facility
348 Chanin Building
1300 Morris Park Avenue
Bronx, New York 10461

J.Paul Robinson, Purdue University Cytometry Labs


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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu