Elite ESP
Rehse, Mark (REHSEMA@cellpro.cellpro.com)
18 Nov 94 09:29:03 PST
This is in response to K. Webber's poll concerning the ESP upgrade. I
have been sorting with the ESP upgrade for about 6 months. We upgraded
our 3 year old Elite when the need to sort rare events became a time
constraint. For the first few months most sorts were a disaster and I
considered retrofitting the old signal processors. However, after going
through the hoops with Coulter and trying matched sets of enclosed
bimorph/flow cell body/flow cell tip QCed in Florida and replacing a
noisy power supply sorting performance began to improve from a "no sort"
condition to 80-90% purity. Several tricks I learned- A) I leave the
sorter powered up 5 days/wk 24 hours/dy with screens dark, fluidics
powered down, sort crystal at 0 % until several hours before a sort. B)
This particular configuration takes 30kHz, 75% Drive, 100% defl., 12 lbs
sheath, drop delay=43.0, sort mode=purity. Drop delay profile comes up
with 18-20 beads/20 beads sorted in drop 43, 0-1 in 42 and 44. Eight to
nine/10 phase settings good. At these settings any change in drop delay
will compromise purity and recovery and so needs almost constant
monitoring. Occasionally a small movement of the aligator clip where it
conects to the flow cell body will "correct" some fanning side streams.
We regularly sort at 7-8,000 events/sec and get 95%+ purities at these
settings. Your particular machine may require slightly different settings
but keep working with it. Hope this helps.
Mark Rehse Rehsema@cellpro.cellpro.com
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