Re: Sorting Biohazards--second try

Dave Coder (dave@nucleus.immunol.washington.edu)
Tue, 15 Nov 94 11:43:31 -0800

Although the normal mode of infection for T. cruzi is via a bug bite, the
trypanasome will easily cross mucus membrane (infection via the conjunctiva
is common). How much of an aerosol will sorting create? How many viable
trypanasomes will be floating around? You should be able to have a well
contained sorting area (negative pressure, properly shielded from the
outside, etc.) BUT, there is no cure for T. cruzi infection: once infected
you have a parasite for life.

So, do you need to sort with a flow cytometer? I'd look at the alternatives first.

Dave Coder
Univ. Washington
dcoder@u.washington.edu

Begin forwarded message:

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Date: Mon, 14 Nov 94 20:48:26 EST
From: (Steve G. Hilliard) <hilliard@zookeeper.zoo.uga.edu>
To: cytometry@flowcyt.cyto.purdue.edu
Subject: Sorting Biohazards--second try

I've had trouble "following my bouncing mail" (can't sing anyway ;-))
but I get the feeling my first attempt didn't make it. To reiterate, I
have a user who wants me to SORT antibody-labelled, VIABLE _T. cruzi_. I'd
appreciate anyone's thoughts--current regulations (if any), precautions,
"don't even consider it", etc. Do we need to put our Elite in a P2 room,
or just forget it entirely? Anyone?

Thanks,
Steve
++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
Steve G. Hilliard, Cell Analysis Facility |
University of Georgia | "Be good and you will
hilliard@zookeeper.zoo.uga.edu | be lonesome..."
++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu