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>Date: Wed, 19 Oct 1994 20:29:54 +0000 (GMT)
>From: HAVILAND@KIDS.WUSTL.EDU (David L. Haviland, Ph.D.)
>Subject: Anti-TCR cross-reactivity?
>To: immunology@net.bio.net
>NNTP-Posting-Host: kids1.wustl.edu
>X-News-Reader: VMS NEWS v1.25
>
>Greetings netters:
>
>I'm afilliated with the Children's Hospital Clinical Immunology lab and I
>have an interesting problem on which I'd like to solicit the groups
>thoughts. We have been expanding our diagnostic flow cytometry panel for T
>and B cells, adhesion molecules, etc... For whatever CD marker we examine,
>we have been very successful in using whole blood for our staining. In the
>analysis, the RBCs/platelet are simply gated out on the basis of size. As
>I said, this works for any "CD-whatever you want" antigen EXCEPT when we
>look specifically at the T-cell receptor (alpha/beta-specifically). Direct
>staining on whole blood doesn't work with this antibody - T cells are
>negative for TCR! Yet, when the lymphocytes are isolated via Ficoll, they
>of course stain for the TCR. I had the (senior) lab tech to do multiple
>gating on *anything* that scatters light (FALS and 90') and it turns out
>that the RBCs were absorbing the TCR MAb. Why?
>
>The only thing I can come up with is some sort of non-specific carbohydrate
>association between the antibody and the variety of carbohydrate moities on
>RBCs. I'm not sure if this thought "holds any water"?
>
>Any thoughts? ...and many thanks in advance.
>David
>===========================================================================
>+ David L. Haviland, Ph.D. Internet:"haviland@kids.wustl.edu" +
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***********************************************
Roger A. Burger E-mail: SL061@cc.usu.edu
Research Immunologist
Center for Persons with Disabilities
Utah State University
Logan, UT 84322-6800
Voice: 801-7502042
FAX: 801-7502044
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