DNA
Joe Lima (jlima@ftzsmmns-amedd.army.mil)
Mon, 19 Sep 94 7:56:35 MST
Recently Overton,W.R and McCoy,J.P. reported in Cytometry 16:351-356 (1994), a
method for reversing the effect of formalin on the binding of Propidium Iodide
to DNA, and more recently presented a poster at the CAC meeting indicating the
same for formalin fixed paraffin embedded tissue.
The method involves the use of thermal treatment (75 degrees for 90 min) to
reverse the effect of formalin on the binding of PI to DNA.
Has anyone else repeated this procedure with similar results.
My first attempt gave only a slight increase in fluorescence intensity.
Any suggestions?
Thanks, joe
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Joe Lima
jlima@ftzsmmns-amedd.army.mil
Fitzsimmons Army Medical Center
Aurora, CO USA
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