Alternatives to dichlorofluorescein
David Hedley (70740.1375@compuserve.com)
17 Sep 94 13:13:18 EDT
I'm starting to do a lot of work on the interrelations between reactive
oxygen intermediates, redox status, and calcium regulation during cell
killing by chemotherapy. Dichlorofluorescein is OK for detecting oxidative
burst in neutrophils, but is too leaky to detect slow rates of generation
of reactive oxygen by redox cycling agents. Also, a probe which picked up
superoxide rather than hydrogen peroxide would be theoretically better.
1) Molecular Probes has a modified form of DCF which is stated to be
better retained than DCF. Does anyone have any experience with it? Is it
any good? (don't mean to be rude, Dick).
2) Dihydrorhodamine 123 is reputed to be more sensitive to superoxide.
Does anyone have good evidence to support this, preferably published
(grants panels and all that).
3) Does DCF respond to free radicals generally, or just to reduced forms
of molecular oxygen? I'm particularly thinking about lipid peroxidation
breakdown products.
David Hedley
Ontario Cancer Institute/Princess Margaret Hospital
Toronto, Canada.
P.S. I got some useful replies to my query about volume measurements. Will
post a summary when all info available.
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