air bubbles
Rochelle A. Diamond (diamond@cco.caltech.edu)
Fri, 9 Sep 1994 08:49:48 -0700 (PDT)
Just thought I would add a contribution to the air bubble discussion. We
(Caltech flow facility) have been purging our sheath fluid for years with
Helium gas and a sparger. I learned to due this when I was working with
HPLC many moons ago. It has worked great especially on those long sorting
runs that last more than 8 hours. It doesn't seem to harm cell viability
or function and we work with all kinds of cells- from mouse thymocytes to
sea urchin coelomocytes. We let the helium sparge for about 15 minutes.
Rochelle Diamond
California Institute of Technology
Flow Cytometry Facility
Pasadena, CA 91125
(818)395-4947
diamond@cco.caltech.edu
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